"There is no question of any alliance with BJP," he told reporters on the sideline of a conference here.
He was responding to a question on the possibility of Biju Janata Dal joining hands with BJP again in the 2014 Lok Sabha polls.
Asked whether there were any chances of revival of the Third Front, Patnaik said, "I think the Third Front is a very healthy option. But it is still early days."
BJD, which has been ruling the eastern state since 1999, had an alliance with BJP till 2009. However, the regional party snapped ties with BJP just before the 2009 Odisha Assembly elections. The party has 14 members in Lok Sabha.
The party was a constituent of Atal Bihari Vajpayee-led NDA government in which Patnaik held a Cabinet berth.