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Ansari will represent India at the independence ceremony in Juba, the capital of the the world`s 193rd country.
The Vice President will also hold a bilateral meeting with Ugandan President Yoweri Museveni in Kampala enroute to South Sudan.
South South had reached a comprehensive peace agreement with North Sudan in 2005, brokered by US Secretary of State Colin Powell under former President George W Bush, that stopped a two-decade-long civil war and paved the way for a referendum in January this year.
India was one of the first Asian countries to open a consulate in Juba in 2007.